Table 2. Estimation of anti-oxidants in cell lysates of IMR-32 cells.
Group | SOD (Units/mg protein) | Catalase (µM H2O2/min/gm protein) | GST (µM/min/gm protein | GPX (µM/min/gm protein |
Control | 9.639±1.42 | 298.85±74.71 | 13.95±1.19 | 532.37±34.8 |
BLM | 9.792±3.46 | 1185.04±27.76* | 33.29±1.67* | 719.07±29.18* |
AA | 11.53±3.45 | 274.84±52.95 | 14.38±3.45 | 732.91±89.15 |
AA+BLM | 20.732±4.28 | 387.05±6.34** | 26.50±6.65 | 1112.67±64.7** |
IMR-32 cells were treated with AA 30 µg/ml and bleomycin 60 µg/ml and incubated for 24 hrs. At the end of treatment period, supernatant was collected and cells were lysed. All values are expressed as mean ± standard error (n = 3). *p<0.05 when compared to control. **P<0.01 when compared to Bleomycin. BLM = Bleomycin, AA = Arachidonic Acid, SOD = Superoxide dismutase, GST = Glutathione-S-transferase, GPX = Glutathione peroxidase.