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. Author manuscript; available in PMC: 2014 Dec 23.
Published in final edited form as: Tissue Antigens. 2008 May 20;72(1):29–38. doi: 10.1111/j.1399-0039.2008.01057.x

Table 1.

Mamu/Mafa/Mane-A2 locus-specific amplification and sequencing primers

Primer ID Annealing Site Sequence (5′-3′) Application
P000044 Sense 5′ UT GATTCTCCGCAGACGCCCA Paired with P000023 for PCR
P000023 Antisense Exon 5 GGAGAACCAGGCCAGCAAT Paired with P000044 for PCR
P000076 Sense Intron 1 GAGCAGCGACGGGACCGCA Exon 2 sequencing (forward)
P000060 Antisense Intron 2 CCTGGGGCTCTCCCGGGTCA Exon 2 sequencing (reverse)
P000096 Sense Intron 2 TGTACTGAGTCTCCCTGATGG Exon 3 sequencing (forward)
P000098 Antisense Intron 3 TTCATTCCCTCAGAGATTTT Exon 3 sequencing (reverse)
P000055 Sense Intron 3 CCCAGGTRCCTSTGTCCAGGA Exon 4 sequencing (forward)
P000294 Antisense Intron 4 CTCTGGGAAAGGAGGGGA Exon 4 sequencing (reverse); Mamu
P000281 Antisense Intron 4 AGAGGGGAAAGTGAGGGGT Exon 4 sequencing (reverse); Mane & Mafa

Note: Unless otherwise specified, primers are useful for all three species