Table 2.
The NLRP1aQ593P mutation reduces the frequency of myeloid progenitor cells in the bone marrow independently of the IL-1R
| Number of colonies | |||||||
|---|---|---|---|---|---|---|---|
| Genotype | Stimulus | Blast | Neutrophil | Granulocyte/Macrophage | Macrophage | Eosinophil | Megakaryocyte |
| Il1r−/− | GM-CSF | 24±8 | 7±2 | 33±17 | 5±5 | ||
| G-CSF | 15±8 | 0±0 | 0±0 | ||||
| M-CSF | 2±2 | 6±6 | 47±18 | ||||
| IL-3 | 4±2 | 27±9 | 11±6 | 23±14 | 3±3 | 7±8 | |
| SCF+IL-6 | 8±3 | 24±5 | 5±3 | 3±1 | 1±1 | 2±2 | |
| Saline | 0±0 | 0±0 | 0±0 | 0±0 | 0±0 | 0±0 | |
| Il1r−/−Nlrp1aQ593P/Q593P | GM-CSF | 13±10 | 3±2* | 8±7* | 1±0 | ||
| G-CSF | 7±2* | 0±0 | 0±0 | ||||
| M-CSF | 0±0* | 0±1 | 8±4* | ||||
| IL-3 | 2±2 | 9±8* | 3±2* | 4±2* | 1±1 | 4±3 | |
| SCF+IL-6 | 3±1* | 10±8* | 1±1* | 2±1* | 0±0 | 2±1 | |
| Saline | 0±0 | 0±0 | 0±0 | 0±0 | 0±0 | 0±0 | |
*
p<0.05, Mean ± SD, n=4–7 independent experiments. 2.5 × 104 bone marrow cells were cultured in the presence of 100 ng/ml murine SCF, 10 ng/mL rm IL-3,10 ng/mL rm GM-CSF, 10 ng/mL rm M-CSF or rh G-CSF in 0.3% agar/DME/FCS. After 7 days, cultures were fixed, stained and counted.