Table 1. Antibodies.
Protein | Source | Clone/Cat. No. | Species | Immunogen | Dilution |
Actin | Sigma | clone AC-15 | Mouse | Slightly modified β-cytoplasmic actin N-terminal peptide | 1∶20,000W |
Calbindin | Sigma | Clone CB-955 | Mouse | purified bovine kidney calbindin-D-28K | 1∶500 |
GFAP | Dako | Clone 6F2 | Mouse | GFAP from human brain | 1∶200f, 1∶1000 |
Glutamine synthetase | BD Transduction | Cat# 610517 | Mouse | Sheep glutamine synthetase aa. 1–373 | 1∶1000f |
Hsp27 | Enzo Life Sciences | Cat# ADI-SPA-801 | Rabbit | recombinant mouse Hsp25 protein | 1∶2500, 1∶400f, 1∶1000W |
Hsp70 | Enzo Life Sciences | Cat# ADI-SPA-810 | Mouse | native human Hsp70 protein | 1∶400, 1∶1000W |
Iba1 | Wako | 019-19741 | Rabbit | Synthetic peptide corresponding to the Iba1carboxy-terminal sequence | 1∶4000f, 1∶20,000 |
NeuN | Millipore | clone A60 | Mouse | Purified cell nuclei from mouse brain | 1∶1000 |
Parvalbumin | Sigma | Clone PARV-19 | Mouse | Purified frog muscle parvalbumin | 1∶2000 |
PCNA | Dako | clone PC10 | Mouse | Rat PCNA-protein A fusion protein obtained from vector pC2T | 1∶2000f |
Np-NFH | Covance | Clone SMI-32 | Mouse | Homogenized hypothalmi recovered from Fischer 344 rats | 1∶10,000 |
χ-synuclein | †DSHB | Cat# CPTC-SNCG-1 (supernatant) | Mouse | recombinant full-length human χ-synuclein | 1∶40f |
vimentin | Dako | clone V9 | Mouse | Purified vimentin from porcine eye lens | 1∶200f |
dilution used for 2-step fluorescent immunostaining procedure;
dilution used for Western blotting; †Developmental Studies Hybridoma Bank.