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. 2014 Dec 23;9(12):e114838. doi: 10.1371/journal.pone.0114838

Table 1. Antibodies.

Protein Source Clone/Cat. No. Species Immunogen Dilution
Actin Sigma clone AC-15 Mouse Slightly modified β-cytoplasmic actin N-terminal peptide 1∶20,000W
Calbindin Sigma Clone CB-955 Mouse purified bovine kidney calbindin-D-28K 1∶500
GFAP Dako Clone 6F2 Mouse GFAP from human brain 1∶200f, 1∶1000
Glutamine synthetase BD Transduction Cat# 610517 Mouse Sheep glutamine synthetase aa. 1–373 1∶1000f
Hsp27 Enzo Life Sciences Cat# ADI-SPA-801 Rabbit recombinant mouse Hsp25 protein 1∶2500, 1∶400f, 1∶1000W
Hsp70 Enzo Life Sciences Cat# ADI-SPA-810 Mouse native human Hsp70 protein 1∶400, 1∶1000W
Iba1 Wako 019-19741 Rabbit Synthetic peptide corresponding to the Iba1carboxy-terminal sequence 1∶4000f, 1∶20,000
NeuN Millipore clone A60 Mouse Purified cell nuclei from mouse brain 1∶1000
Parvalbumin Sigma Clone PARV-19 Mouse Purified frog muscle parvalbumin 1∶2000
PCNA Dako clone PC10 Mouse Rat PCNA-protein A fusion protein obtained from vector pC2T 1∶2000f
Np-NFH Covance Clone SMI-32 Mouse Homogenized hypothalmi recovered from Fischer 344 rats 1∶10,000
χ-synuclein DSHB Cat# CPTC-SNCG-1 (supernatant) Mouse recombinant full-length human χ-synuclein 1∶40f
vimentin Dako clone V9 Mouse Purified vimentin from porcine eye lens 1∶200f
f

dilution used for 2-step fluorescent immunostaining procedure;

W

dilution used for Western blotting;Developmental Studies Hybridoma Bank.