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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Brain Behav Immun. 2014 Oct 12;44:195–206. doi: 10.1016/j.bbi.2014.09.015

Figure 2.

Figure 2

Effects of the 6-cycles of RSD on mRNA expression of chromatin modification-associated proteins and status of DNA methylation in splenic macrophages (CD11b+ cells).

(A) mRNA expression of DNA methyltransferases DNMT3a, DNMT3b, and DNMT1 in the control (clear bar) and RSD (filled bar) groups (mean ± SEM, n = 8/group)

(B) mRNA expression of histone deacetylase 2 (mean ± SEM)

(C) Standard curve plot for OD values observed at 450 nm vs. concentrations of control methylated DNA (5-mC)

The coefficient of determination (R2) for the linear regression is 0.9947.

(D) The absolute amount of methylated DNA in splenic macrophages (CD11b+ cells) in control and RSD groups (mean ± SEM)

*: p< 0.001; **: p< 0.0001