DNA content (A), GAG content (B), total collagen content (C), and ALP activity (D) for the hMSCs encapsulated in Superficial (L1, blue), Middle (L2, red), and Calcified (L3, green) gels with incubation time in chondrogenic medium supplemented with zone-specific growth factors. A star indicates a statically significant difference between the test and all other groups at each time point. Error bars correspond to means±1 SD for n=3.