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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Integr Biol (Camb). 2015 Jan;7(1):112–127. doi: 10.1039/c4ib00197d

Table 1.

Gel composition and degradation time, matrix stiffness, type, concentration and exposure time of growth factors, and hMSC cell density for simulating superficial, middle, and calcified zones of articular cartilage.

Simulated
cartilage
zone		 Gel composition
and degradation
time		 Matrix
stiffness		 Growth
factors
(GF)		 GF
Exposure
time		 Cell
density
(cells/mL)
Superficial SPELA10 (15%)
4weeks		 80kPa/ TGFβ1
(3 ng/mL)

BMP-7
(100 ng/mL)		 10 days 60×106
Middle SPELA7.5 (50%)
8 weeks		 2.1 MPa/ TGFβ1
(30 ng/mL)

IGF-1
(100 ng/mL)		 21 days 20×106
Calcified PLA/SPELA5 (35%)
fiber composite
3% HA
12 weeks		 320 MPa/ TGFβ1
(30 ng/mL)		 21 days 15×106