Skip to main content
PMC home page
Proceedings of the National Academy of Sciences of the United States of America logoLink to Proceedings of the National Academy of Sciences of the United States of America
. 1972 Jan;69(1):20–24. doi: 10.1073/pnas.69.1.20

Detection of Avian Tumor Virus-Specific Nucleotide Sequences in Avian Cell DNAs

Harold E Varmus *, Robin A Weiss †,‡, Robert R Friis †,‡, Warren Levinson *, J Michael Bishop *
PMCID: PMC427535  PMID: 4333039

Abstract

The effect of unlabeled cellular DNA upon the reassociation kinetics of labeled double-stranded DNA made by DNA polymerase from avian tumor viruses has been used to measure virus-specific nucleotide sequences in cells. Multiple copies of these sequences were found equally in normal chick cells, in chick cells transformed in culture, and in Rous tumor cells. Copies were also present equally in cells harboring chick helper factor and group-specific antigen, and in cells lacking those characteristics. Quail DNA also contains multiple copies of these sequences, but no copies were detected in HeLa-cell DNA or salmon-sperm DNA.

Keywords: reassociation kinetics, RNA tumor viruses, gs antigen, Rous sarcoma virus, chick cells

Full text

PDF
20

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. BERNS K. I., THOMAS C. A., Jr ISOLATION OF HIGH MOLECULAR WEIGHT DNA FROM HEMOPHILUS INFLUENZAE. J Mol Biol. 1965 Mar;11:476–490. doi: 10.1016/s0022-2836(65)80004-3. [DOI] [PubMed] [Google Scholar]
  2. Bentvelzen P., Daams J. H., Hageman P., Calafat J. Genetic transmission of viruses that incite mammary tumor in mice. Proc Natl Acad Sci U S A. 1970 Sep;67(1):377–384. doi: 10.1073/pnas.67.1.377. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bishop J. M., Koch G. Infectious replicative intermediate of poliovirus: purification and characterization. Virology. 1969 Apr;37(4):521–534. doi: 10.1016/0042-6822(69)90270-0. [DOI] [PubMed] [Google Scholar]
  4. Bishop J. M., Levinson W. E., Quintrell N., Sullivan D., Fanshier L., Jackson J. The low molecular weight RNAs of Rous sarcoma virus. I. The 4 S RNA. Virology. 1970 Sep;42(1):182–195. doi: 10.1016/0042-6822(70)90251-5. [DOI] [PubMed] [Google Scholar]
  5. Britten R. J., Kohne D. E. Repeated sequences in DNA. Hundreds of thousands of copies of DNA sequences have been incorporated into the genomes of higher organisms. Science. 1968 Aug 9;161(3841):529–540. doi: 10.1126/science.161.3841.529. [DOI] [PubMed] [Google Scholar]
  6. Fanshier L., Garapin A. C., McDonnell J., Faras A., Levinson W., Bishop J. M. Deoxyribonucleic acid polymerase associated with avian tumor viruses: secondary structure of the deoxyribonucleic acid product. J Virol. 1971 Jan;7(1):77–86. doi: 10.1128/jvi.7.1.77-86.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Garapin A. C., Fanshier L., Leong J. A., Jackson J., Levinson W., Bishop J. M. Deoxyribonucleic acid polymerases of Rous sarcoma virus: kinetics of deoxyribonucleic acid synthesis and specificity of the products. J Virol. 1971 Feb;7(2):227–232. doi: 10.1128/jvi.7.2.227-232.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Gelb L. D., Aaronson S. A., Martin M. A. Heterogeneity of murine leukemia virus in vitro DNA; detection of viral DNA in mammalian cells. Science. 1971 Jun 25;172(3990):1353–1355. doi: 10.1126/science.172.3990.1353. [DOI] [PubMed] [Google Scholar]
  9. Gelb L. D., Kohne D. E., Martin M. A. Quantitation of Simian virus 40 sequences in African green monkey, mouse and virus-transformed cell genomes. J Mol Biol. 1971 Apr 14;57(1):129–145. doi: 10.1016/0022-2836(71)90123-9. [DOI] [PubMed] [Google Scholar]
  10. Green M. Oncogenic viruses. Annu Rev Biochem. 1970;39:701–756. doi: 10.1146/annurev.bi.39.070170.003413. [DOI] [PubMed] [Google Scholar]
  11. Hanafusa H., Miyamoto T., Hanafusa T. A cell-associated factor essential for formation of an infectious form of Rous sarcoma virus. Proc Natl Acad Sci U S A. 1970 Jun;66(2):314–321. doi: 10.1073/pnas.66.2.314. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hanafusa T., Hanafusa H., Miyamoto T. Recovery of a new virus from apparently normal chick cells by infection with avian tumor viruses. Proc Natl Acad Sci U S A. 1970 Dec;67(4):1797–1803. doi: 10.1073/pnas.67.4.1797. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Harel L., Harel J., Lacour F., Huppert J. Homologie entre génome du virus de la myéloblastose aviare (AMV) et génome cellulaire. C R Acad Sci Hebd Seances Acad Sci D. 1966 Aug 8;263(6):616–619. [PubMed] [Google Scholar]
  14. Huebner R. J., Kelloff G. J., Sarma P. S., Lane W. T., Turner H. C., Gilden R. V., Oroszlan S., Meier H., Myers D. D., Peters R. L. Group-specific antigen expression during embryogenesis of the genome of the C-type RNA tumor virus: implications for ontogenesis and oncogenesis. Proc Natl Acad Sci U S A. 1970 Sep;67(1):366–376. doi: 10.1073/pnas.67.1.366. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Huebner R. J., Todaro G. J. Oncogenes of RNA tumor viruses as determinants of cancer. Proc Natl Acad Sci U S A. 1969 Nov;64(3):1087–1094. doi: 10.1073/pnas.64.3.1087. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Levinson W., Bishop J. M., Quintrell N., Jackson J. Presence of DNA in Rous sarcoma virus. Nature. 1970 Sep 5;227(5262):1023–1025. doi: 10.1038/2271023a0. [DOI] [PubMed] [Google Scholar]
  17. Payne L. N., Chubb R. C. Studies on the nature and genetic control of an antigen in normal chick embryos which reacts in the COFAL test. J Gen Virol. 1968 Dec;3(3):379–391. doi: 10.1099/0022-1317-3-3-379. [DOI] [PubMed] [Google Scholar]
  18. Rosenthal P. N., Robinson H. L., Robinson W. S., Hanafusa T., Hanafusa H. DNA in uninfected and virus-infected cells complementary to avian tumor virus RNA. Proc Natl Acad Sci U S A. 1971 Oct;68(10):2336–2340. doi: 10.1073/pnas.68.10.2336. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. TEMIN H. M. HOMOLOGY BETWEEN RNA FROM ROUS SARCOMA VIROUS AND DNA FROM ROUS SARCOMA VIRUS-INFECTED CELLS. Proc Natl Acad Sci U S A. 1964 Aug;52:323–329. doi: 10.1073/pnas.52.2.323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. TEMIN H. M. THE EFFECTS OF ACTINOMYCIN D ON GROWTH OF ROUS SARCOMA VIRUS IN VITRO. Virology. 1963 Aug;20:577–582. doi: 10.1016/0042-6822(63)90282-4. [DOI] [PubMed] [Google Scholar]
  21. Varmus H. E., Levinson W. E., Bishop J. M. Extent of transcription by the RNA-dependent DNA polymerase of Rous sarcoma virus. Nat New Biol. 1971 Sep 1;233(35):19–21. doi: 10.1038/newbio233019a0. [DOI] [PubMed] [Google Scholar]
  22. Vogt P. K., Friis R. R. An avian leukosis virus related to RSV(O): properties and evidence for helper activity. Virology. 1971 Jan;43(1):223–234. doi: 10.1016/0042-6822(71)90240-6. [DOI] [PubMed] [Google Scholar]
  23. Wetmur J. G., Davidson N. Kinetics of renaturation of DNA. J Mol Biol. 1968 Feb 14;31(3):349–370. doi: 10.1016/0022-2836(68)90414-2. [DOI] [PubMed] [Google Scholar]
  24. Yoshikawa-Fukada M., Ebert J. D. Hybridization of RNA from Rous sarcoma virus with cellular and viral DNA's. Proc Natl Acad Sci U S A. 1969 Nov;64(3):870–877. doi: 10.1073/pnas.64.3.870. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Proceedings of the National Academy of Sciences of the United States of America are provided here courtesy of National Academy of Sciences

RESOURCES