Figure 3. PHT conditioned medium inhibits HIV infection.

(A) Relative luciferase activity (RLU) of an HIV Tat-inducible reporter in TZM-bl cells exposed to either conditioned PHT or control non-conditioned medium and infected with increasing concentrations of HIV, as described in Methods. Data are representative of three experiments, each performed in triplicate. * denotes p <0.05, and ** denotes p <0.001 (Student's t test). (B) Relative luciferase expression in TZM-bl cells transfected with miR-517-3p, miR-720, or control scrambled mimic and infected with increasing concentrations of HIV. Data are representative of three experiments, each performed in triplicate. None of the differences were statistically significant.