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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Traffic. 2014 Nov 14;16(1):48–67. doi: 10.1111/tra.12234

Figure 7. Role of recycling proteins in regulating inter-kinetochore tension.

Figure 7

(A-D) Cells were transfected with indicated siRNAs for 48 h, fixed and stained with anti-centromere/kinetochore antisera (CREST, green) and tubulin (red) antibody. A single 0.2 μm optical section is shown for each condition. (E) Inter-kinetochore distance was measured in >100 kinetochores from 10 representative cells across three experiments. Asynchronous HeLa cells (F and G) or BJ human fibroblasts (H and I) were subjected to Triton X-100 extraction prior to fixation (see methods) and then stained with anti-MICAL-L1 antibody (green) and DAPI (blue). Maximum projections of 1 μm optical sections are shown. One-way ANOVA **p<0.01.