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. Author manuscript; available in PMC: 2015 Oct 23.
Published in final edited form as: Oncogene. 2014 Jun 23;34(17):2178–2188. doi: 10.1038/onc.2014.177

Figure 3. β-catenin expression requires mTOR, HSF1 and HuR expression.

Figure 3

(A) MDAMB-231 HSF1, mTOR and HuR knockdown cells were generated with shRNA expressing lentivirus specific for each targeted mRNA. Expression levels of HuR, β-catenin, HSF1 phospho-S326, total HSF1, phospho S6K p70, total S6K p70 were measured by Western blotting and quantitated by densitometry (right side). Levels of actin were also measured as loading controls. (B) Protein levels of HSF1, β-catenin and HuR were measured in control and HSF1 knocked down MCF7 and MCF10A cells. β-actin was also measured using Western Blotting. Bands in gels were measured by densitometric analyses using Image J software and shown in right side. The experiment was carried out in duplicate with reproducible results.