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. Author manuscript; available in PMC: 2015 Oct 23.
Published in final edited form as: Oncogene. 2014 Jun 23;34(17):2178–2188. doi: 10.1038/onc.2014.177

Figure 5. Constitutive expression of HuR overcomes the effects of mTOR knockdown on β-catenin depletion.

Figure 5

mTOR concentration was reduced by expression of siRNA in MDA-MB-231 cells (control and 1A3-HuR overexpressing cells). Expression levels of HuR, β-catenin, total HSF1, phospho S6K p70, total S6K p70 and mTOR were measured by Western blotting and quantitated by densitometry (shown below).Actin levels were also measured for loading control. These procedures were performed twice with reproducible results.