Skip to main content
. Author manuscript; available in PMC: 2015 Oct 30.
Published in final edited form as: Oncogene. 2014 Jun 23;34(18):2325–2336. doi: 10.1038/onc.2014.173

Figure 3. Dek expression supports breast cancer metastasis in vivo and in vitro.

Figure 3

(A) RontgDek+/+ mice have a greater metastatic lung tumor burden than RontgDek−/−. Lungs were harvested from six RontgDek−/− and five RontgDek+/+ mice with similar tumor volumes for the largest tumor and serial sections were microscopically analyzed for tumor metastases. Total tumor burden from all lung sections in wild type mice was 18.38 ± 1.754 compared to 8.183 ± 1.661 in knockout mice (p= 0.0023, unpaired two-tailed t-test). The average number of lung metastases per mouse is quantified and a representative H&E from each genotype is depicted below. (B) Dek expression correlates with cellular invasion in vitro. RontgDek+/+ cell lines (R7 and RD258) transduced with Deksh2 (“+Deksh2”) or NTsh control, and RontgDek−/− cell lines (RD147, RD219, RD238 and RD271) transduced with a mDek construct (“+mDek”) or control R780 vector were subjected to Matrigel transwell assays. Data is represented as fold change compared to the respective control for each cell line (NTsh or mDek) for triplicate experiments. Significance was calculated with a one-tailed unpaired t-test.