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. Author manuscript; available in PMC: 2014 Dec 23.
Published in final edited form as: Methods Mol Biol. 2012;798:475–490. doi: 10.1007/978-1-61779-343-1_28

Fig. 2.

Fig. 2

Luciferase reporter assays of miR-1 and HDAC4-3′ UTR. (a) Graphic representation of the modified luciferase vector containing the 3′ UTR of the HDAC4 gene. (b) Sequence comparison among HDAC4 wild-type 3′ UTR, HDAC4 mutant 3′ UTR sequence, and miR-1. (c) miR-1 significantly represses the luciferase reporter activity of HDAC4 wild-type 3′ UTR, but not that of mutant 3′ UTR.