Figure 3.

Fungal-specific blastogenesis of peripheral blood (PB) (A and C) CD3⁺CD4⁺ cells and (B and D) CD3⁺CD8⁺ cells in a (A and B) chronic rhinosinusitis with nasal polyp (CRSwNP) and (C and D) an allergic fungal rhinosinusitis (AFRS) patient. Carboxyfluorescein succinimidyl ester (CFSE)–labeled peripheral blood mononuclear cells were cultured with no mitogen (unstimulated), phytohemagglutinin (PHA), and Alternaria alternata fungal antigens. Cells were harvested after 6 days of the proliferative period. Dot plots are gated on CD3⁺ blast cells (please also refer to Fig. 2). Both CD4⁺ and CD8⁺ T cells divided in response to fungal antigens in the CRSwNP patient. In contrast, CD8⁺ T cells did not divide to fungal antigens in the AFRS patient.