Table 1.

The primer sequences, exons amplified and the restriction enzymes used in the PCR/RFLP procedures to detect five G6PD deficient mutations and the silent one

Variant	Primers	Exon amplified	Restriction Enzyme	Refs.
Mediterranean (563 C→T)	5′ GGT GAG GCT CCT GAG TAC CA 3′ 5′ AGC TGT GAT CCT CAC TCC CC 3′	VI	MboII	[13]
Chatham (1003 G→A)	5′ CAA GGA GCC CAT TCT CTC CCT T 3′ 5′ TTC TCC ACA TAG AGG AGG ACG GCT GCC AAA GT 3′	IX	BstXI	[14]
A-(202 G→A)	5′ CGT GTC CCC AGC CAC TTC TA 3′ 5′ CAC GCT CAT AGA GTG GTG GG 3′	III–V	NlaIII	[14]
Cosenza (1376 G→C)	5′ GCA GCC AGT GGG ATC AGC AAG 3′ 5′ GGC AAG GAG GGT GGC CGT GG 3′	XI–XIII	Bsu361	[14]
Silent (1311 C→T)	5′ TGT TCT TTC AAC CCC GAG GAG T 3′ 5′ AAG ACG TCC AGG ATG AGG TGA TC 3′	X–XI	Bcl I	[15]