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. 2014 Dec 8;3(12):e131. doi: 10.1038/oncsis.2014.47

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Copyright © 2014 Macmillan Publishers Limited

Oncogenesis is an open-access journal published by Nature Publishing Group. This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-nd/4.0/

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SUV39H1 decreases cleaved poly-ADP ribose polymerase (cPARP) in sulforaphane-treated PC3 cells. PC3 cells were transfected with GFP (control) or SUV39H1 (SUV) expression vector and allowed to recover for 36 h. Cells were incubated with sulforaphane for 24 h before collecting whole-cell lysates. H3K9me3 is increased in SUV39H1-transfected PC3 cells (SUV: dimethyl sulfoxide (DMSO), SUV:SFN) relative to GFP control (GFP:DMSO, GFP:SFN) (normalized to histone H3), although no increase in total SUV39H1 protein was noted (normalized to β-actin). Sulforaphane treatment significantly increased apoptotic signaling as assessed by cPARP in GFP- and SUV39H1-transfected cells. SUV39H1 overexpression significantly decreased cPARP protein levels, indicating decreased apoptotic signaling (*P<0.05, **P<0.01 by one-way analysis of variance with Bonferroni post test for each protein, mean+s.e.m. for two independent experiments; blot from representative experiment pictured).