Fig. 5.

50% in vivo inhibition of luciferase activity achieved via topical delivery of Accell^™-siRNAs using a novel siRNA-ointment formulation. (a) 40 µg of the Aquaphor^®-PG-siRNA (native or Accell^™) was applied to the paws of FLG-luc2p^+/− mice (3/group) for 50 min; treatments were repeated every 24 h for 5 days (Days 0-4; noted with *). Left paws were treated with siLUC2P-2 (300 pmol) and right paws with NSC4 (300 pmol). Control group received the Aquaphor^®-PG without siRNA on the left paw and no treatment on the right paw. Representative images are shown here (see Supplementary Fig. 9 for full dataset). (b) Graph depicts the average %L/R ratio for each treatment group over the 9-day time-course.