Figure 5. The imino–imino region of ¹⁵N-edited and ¹⁵N/¹³C-filtered NOESY spectrum.

Data were acquired on a sample that contains an equimolar mixture of unlabelled and uniformly ¹³C, ¹⁵N-GC-labelled VK1 oligonucleotides. The spectrum was recorded at 0.9 mM concentration per strand, 0 °C, pH 6 and mixing time of 100 ms. Assignment of individual cross-peaks is indicated. The position of a diagonal in a corresponding standard ¹H–¹H NOESY spectrum is shown as a straight line.