Table 1. X-ray crystallographic statistics of IL-18 and its extracellular complexes.
IL-18 | IL-18/IL-18Rα | IL-18/IL-18Rα/IL-18Rβ | |
---|---|---|---|
Data collection | |||
Beamline | BL38B1 (SPring-8) | BL44XU (SPring-8) | BL17A (Photon Factory) |
Wavelength | 1.0000 | 0.9000 | 0.9800 |
Space group | P21 | P21212 | P212121 |
Cell dimensions | |||
a, b, c (Å) | 68.15, 79.51, 73.46 | 135.49, 174.81, 183.40 | 72.56, 111.56, 134.57 |
α, β, γ (°) | 90.00, 100.97, 90.00 | 90.00, 90.00, 90.00 | 90.00, 90.00, 90.00 |
Resolution (Å) | 45.0–2.33 (2.46–2.33) | 43.9–3.10 (3.27–3.10) | 50.0–3.10 (3.21–3.10) |
Rmerge | 4.9 (39.6) | 10.0 (58.2) | 11.5 (68.3) |
I/σI | 20.5 (3.6) | 14.9 (2.5) | 19.8 (2.9) |
Completeness (%) | 97.8 (97.1) | 99.6 (100) | 99.9 (99.3) |
Redundancy | 3.8 (3.8) | 3.7 (3.8) | 9.8 (9.2) |
Refinement | |||
Resolution (Å) | 34.82–2.33 (2.41–2.33) | 42.00–3.10 (3.18–3.10) | 42.00–3.10 (3.29–3.10) |
No. reflections | 32,157 (2,861) | 79,130 (5,821) | 17,368 (1,825) |
Rwork/Rfree | 21.8/27.1 (27.6/29.8) | 19.1/22.2 (22.7/26.3) | 18.8/23.2 (20.9/29.7) |
No. atoms | |||
Protein | 5,022 | 20,533 | 5,663 |
Glycan | — | 1,225 | 335 |
Others | 711 | 289 | 32 |
B-factors | |||
Protein | 46.13 | 78.31 | 74.38 |
Glycan | — | 121.43 | 139.74 |
Others | 60.94 | 109.02 | 36.55 |
R.m.s. deviations | |||
Bond lengths (Å) | 0.010 | 0.009 | 0.009 |
Bond angles (°) | 1.38 | 1.11 | 1.17 |
Ramachandran plot | 95.2, 4.8 | 94.4, 5.0, 0.5 | 93.7, 5.4, 0.8 |
Values for Ramachandran plots are presented as favoured, allowed, outlier examined by RAMPAGE. Data collection statistics were summarized from the report35.