Table 2. Functional epitopes of IL-18 and IL-18 receptors.
| Kd (10−8 M) | |
|---|---|
| Ligand: IL-18Rβ WTAnalyte: IL-18 mutant/IL-18Rα WT complex | |
| IL-18 WT(control) | 1.5±0.1 |
| IL-18 G108A | No binding |
| IL-18 H109A | No binding |
| IL-18 D110A | 5.6±0.8 |
| IL-18 K112A | No binding |
| IL-18 G145A | 2.8±0.1 |
| IL-18 R147A | 5.7±0.3 |
| IL-18 M150A | 6.0±0.5 |
| Ligand: IL-18Rβ mutantAnalyte: IL-18 WT/IL-18Rα WT complex | |
| IL-18Rβ WT (control) | 1.5±0.1 |
| IL-18Rβ L167A | 3.3±0.4 |
| IL-18Rβ E210A | 32.7±0.9 |
| IL-18Rβ Y212A | No binding |
| IL-18Rβ Y214A | 5.0±0.5 |
| IL-18Rβ K313A | 10.9±0.9 |
| Ligand: IL-18Rβ WTAnalyte: IL-18WT/IL-18Rα mutant complex | |
| IL-18Rα WT (control) | 1.5±0.1 |
| IL-18Rα N236Q | 1.7±0.3 |
| Ligand: IL-18Rα mutantAnalyte: IL-18 WT | |
| IL-18Rα WT (control) | 6.9±0.2 |
| IL-18Rα N297Q | 25.8±2.6 |
Ligand: the interactant captured on the sensor surface. Analyte: the interactant in free solution. WT: wild type. Values are the means±s.d. of the results derived from three independent experiments.