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. 2015 Feb 6;5(1):20140061. doi: 10.1098/rsfs.2014.0061

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© 2014 The Authors. Published by the Royal Society under the terms of the Creative Commons Attribution License http://creativecommons.org/licenses/by/4.0/, which permits unrestricted use, provided the original author and source are credited.

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Figure 2. — Functional genomics tools for ascidians. (a) ANISEED database. Screenshot of the gene expression interface, depicting palp expression of three different genes at the hatching larva (stage 25) of C. intestinalis. (b) Transient transgenesis by electroporation, available tools and approach. Tissue-specific drivers are regulatory regions of genes expressed in restricted regions of the embryo. They can be used to either drive reporter genes (LacZ in blue) for the analysis of upstream regulatory signatures or can be recombined with coding regions (ORFs) of candidate genes for the analysis of individual candidate genes (a), or for simultaneous testing of small groups of genes (b). Overexpression in palps precursors (in ectoderm, using pFOG, pFT or anterior ectoderm using pDMRT, pFoxC) could give insights into the contribution of the tested genes in adhesive organ formation. The GATEWAY cloning context (Invitrogen) was used for generating a vector suite [44] that allows efficient recombination of drivers and full ORF cDNA clones from arrayed genome wide clone collections [45].