Figure 1.

Schematic overview of our library architecture. After library preparation is finished with a PCR using the PreHyb primer pair, short, barcoded libraries can be used for, e.g. target enrichment via hybridization. Adapter sites of such short libraries need to be completed via indexing PCR before sequencing. If different experiments from the same libraries, such as mitochondrial target enrichment and shotgun sequencing, are pooled for sequencing, sequences from those experiments can be differentiated by the index sequences. The four regular Illumina sequencing primer sites for MiSeq, HiSeq and NextSeq instruments are shown, only the i5 index primer* is different for the NextSeq500.