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Toxicity results in vitro. (A) EC50 values from MTT assay using silver nanoparticles with different coatings and HepG2 cells; (B) Analogous with Neuro-2 cells; (C) Detection of oxidative stress from Ag-NPs (concentration 5 pM) via formation of protein carbonyls incubated with HepG2 cells. (D) Protein carbonyls were detected at different concentrations (2.5, 5, 10 pM) as (DNP) hydrazone adducts via immunoblots with a DNP antibody.
