. 2014 Dec 16;14:269. doi: 10.1186/s12870-014-0269-4

Table 2.

Construct design for enzymatic assays in E. coli

Genes to be analysed	Plasmids contained in *E. coli* (BL21) and the carotenoid ^a being produced (in parentheses)
Genes to be analysed	Negative control	Functional assays	Positive control
LbPSY1/LrPSY1	pACCRT-E + pEASY-E1	pACCRT-E + pEASY-LbPSY1 or pACCRT-E + pEASY-LrPSY1	pACCRT-EB
LbPSY1/LrPSY1	(GGPP)	pACCRT-E + pEASY-LbPSY1 or pACCRT-E + pEASY-LrPSY1	(phytoene)
LbCYC-B/LrCYC-B	pACCRT-EIB + pEASY-E1	pACCRT-EIB + pEAST-LbCYC-B or pACCRT-EIB + pEASY-LrCYC-B	pACCAR16ΔcrtX
LbCYC-B/LrCYC-B	(lycopene)		(β-carotene)
LbCRTR-B2/LrCRTR-B2	pACCAR16ΔcrtX + pEASY-E1	pACCAR16ΔcrtX + pEASY-LbCRTR-B2 or pACCAR16ΔcrtX + pEASY-LrCRTR-B2	pACCAR25ΔcrtX
LbCRTR-B2/LrCRTR-B2	(β-carotene)		(zeaxanthin)

^aGGPP is an exception.