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. 2014 Nov 15;7(11):3933–3940.

Figure 2.

Figure 2

A. Expression of HIF-1α in MDA-MB-231 cells transfected with random siRNA or HIF-1α-targeted siRNA. After serum starvation for 12 h, total cellular protein was extracted and the protein level of HIF-1α was detected using Western blot. β-tubulin was used as internal reference. B. Growth curves of MDA-MB-231 cells transfected with random siRNA or HIF-1α-targeted siRNA. Cells were seeded in 24-well plates and cultured for 9 days. Cell numbers of four wells were counted every other day for each group. The average cell numbers were used to plot the growth curve (n = 3). The data were expressed as means ± SEM. *, P < 0.05 compared with control at respective time point.