Table 1.

Effect of pivaloylacylation-7ADCA on revertant colonies in Ames assay

Dose (μg/plate)	The revertant number/plate (X̅±S)
	TA97		TA98		TA100		TA102
	-S9	+S9	-S9	+S9	-S9	+S9	-S9	+S9
0	120.7±15.1	118.9±13.9	36.7±5.8	36.8±6.0	142.6±18.1	142.1±15.4	254.2±13.8	255.2±14.1
8	112.5±13.9	113.4±11.8	33.1±5.9	34.2±5.2	136.8±16.4	140.6±14.1	252.5±18.6	248.4±14.9
40	120.0±18.4	115.5±15.4	30.9±6.3	35.1±7.0	136.7±10.5	137.4±11.0	244.2±18.1	250.0±14.3
200	114.7±16.2	107.0±11.4	30.8±10.8	31.4±10.3	134.6±18.7	133.4±13.4	263.6±13.4	250.2±12.3
1000	115.9±17.9	115.6±15.8	35.5±9.0	33.1±11.6	133.5±12.8	140.4±11.6	247.5±17.6	253.4±12.7
5000	118.2±15.1	114.3±14.8	35.0±10.0	36.2±10.2	136.0±14.9	128.8±13.9	256.4±17.0	251.7±12.1
Solvent control	115.3±14.3	114.6±12.2	34.1±9.0	34.1±10.3	144.2±15.1	144.3±12.9	251.2±11.2	252.6±11.5
Positive control	1122.8±148.1a	1090.5±121.7a	875.9±109.8a	852.8±112.3a	1135.6±116.5a	1085.1±107.2a	1260.6±97.4a	1105.6±98.9a

The mutagenicity of pivaloylacylation-7ADCA was firstly assessed by Ames assay. Four test strains (TA97, TA98, TA100 and TA102) were exposed to pivaloylacylation-7ADCA, along with solvent control 1‰ (ν/ν) DMSO and positive controls and incubated for 48 h. The positive controls contained 2-aminofluorene (2-AF, 10 μg/plate vs. TA97, TA98, TA100 with S9), 1,8-dihydroxyanthraquinone (50 μg/plate vs. TA102 with S9), 2, 4, 7-trinitrofluoren-9-one (2,4,7-tNFO, 0.2 μg/plate vs. TA97, TA98 without S9), sodium azide (SA, 1.5 μg/plate vs. TA100 without S9), mitomycin C (MMC, 0.5 μg/plate vs.TA102 without S9). Data were obtained from three independent experiments and were expressed as mean ± standard deviation (S.D.).

^adenotes more than 2-fold increase in the number of revertant colonies of the negative control.

Statistical significance was set as P<0.05