Figure 5.

miR-155 protected grafts from rejection in vivo by impacting DCs maturation. (A) Mice i.v injected with AantagomiR-155 (100 nM) or its negative ctrl (NC). After indicated time, mice were sacrificed, and heart, PBMC and spleen were harvested to measure miR-93 expression. (B) Mice were treated as in (A), grafts (BALBc to C57BL/6) survival was evaluated for 12 days (Mann-Whitney test, n = 10). (C, D) Mice were treated as in (A), after four days PBMC, spleens and lymph nodes were harvested, then CD11c cells were sorted from PBMC or tissue suspension. SOCS1 protein expression was measured by western blot and CD40, CD80, CD86 mRNA level was detected by RT-PCR. Data are shown as the mean ± SD. (n = 3) (A, D) or representative of three separate experiments (C) with similar results. **p < 0.01.