Table 3.
Expression of MT isoform mRNAs in Zinc Treated RWPE-1 Cellsa
| MT Isoformb | mRNA Expressionc |
|---|---|
| MT-1A | 0.00519 |
| MT-1E | 57.2 |
| MT-1F | 0.534 |
| MT-1G | 0.581 |
| MT-1H | 0.0384 |
| MT-1X | 3.05 |
| MT-2A | 1.57 |
| MT-3 | 0.000189 |
a
Messenger RNA expression was measure with real time PCR using MT isoform specific primers and total RNA extracted from RWPE-1 cells treated with 75 μM zinc for four days (see the section “Real Time Analysis of MT isoform mRNA Expression” at page 8).
b
Other isoforms were measured but not detected, MT-1B, -1I, -1J, -1K, -1L, and MT-4. The expression of the rest three MT isoforms, MT-1C, -1D and -1M, was not assessed since their gene sequences are either not available in databases (e.g. NCBI or Swiss-Prot) or of controversy.
c
Expressed as the number of mRNA molecules normalized to that of the house-keeping gene, glyceraldehyde-3 phosphate dehydrogenase.