Figure 4.
Effect of temperature on the diffusion coefficient of BODIPY FL-C12. E. coli DH5α cells grown at 37°C were labelled with the green fluorescent lipid analogue BODIPY FL-C12 in the cytoplasmic membrane. Cells were equilibrated to the temperatures shown before measuring the diffusion coefficient by FRAP as described in Experimental procedures. The mean diffusion coefficient at each temperature is shown (n = 10, ± SD). The sigmoidal fit indicates a phase transition melting temperature of 28°C.