Figure 5.
Effect of growth and measurement temperatures on the lateral diffusion of lipids and proteins. Mean diffusion coefficients for BODIPY FL-C12 and two GFP-tagged membrane proteins are shown (n = 10, ± SD).
A. E. coli cells were grown at the temperatures indicated, and lateral diffusion coefficients measured by FRAP at the same growth temperature.
B. Cells were grown at 37°C and lateral diffusion coefficients measured at the temperatures indicated. Time required for sample preparation and equilibration at measurement temperature was about 20 min.