Table 1.
Commonly Used Antibody Clones and Dilutions for the Identification and Isolation of Adipose Tissue Cell Populations.
| Antibody | Gene Name | Cell Population | Supplier | Clone | Flurochrome | Dilution* | Excitation (nm) | Bandpass Filter |
|---|---|---|---|---|---|---|---|---|
| Longpass Filter | ||||||||
| Detected Wavelengths | ||||||||
| CD45 | Leukocyte common antigen | Blood | eBioscience | 30-F11 | APC-eFluor e780 | 1:5000 | 640nm (Red) | 780/60 |
| 755 LP | ||||||||
| 755–810 nm | ||||||||
| CD31 | Pecam1; platelet/endot helial cell adhesion molecule 1 | Endothelial | eBioscience | 390 | PE-Cy7 | 1:1200 | 532nm (Green) | 780/60 |
| 735 | ||||||||
| 750 – 810 nm | ||||||||
| CD34 | cluster differentiation hematopoietic progenitor cell antigen | Mesenchymal stem cells | Biolegend | MEC 14.7 | Alexa Fluor 647 | 1:200 | 640nm (Red) | 660/20 |
| N/A | ||||||||
| 650–670 nm | ||||||||
| CD29 | Integrin β1 | Biolegend | HMB eta1-1 | Alexa Fluor 700 | 1:400 | 640nm (Red) | 710/50 | |
| 690 LP | ||||||||
| 690 – 735 nm | ||||||||
| ScaI | Stem cell antigen-1 or Ly6A/E | BD Horizon | D7 | V450 | 1:1000 | 405nm (Violet) | 450/50 | |
| N/A | ||||||||
| 425–475 nm | ||||||||
| CD24 | Heat-Stable Antigen, HSA or HsAg | BD Bioscience | M1/69 | PE | 1:100 | 532nm (Green) | 575/26 | |
| N/A | ||||||||
| 562–588 nm | ||||||||
| PerCP-Cy5.5 | 1:100 | 532nm (Green) | 710/50 | |||||
| 685 LP | ||||||||
| 685–735 nm | ||||||||
| PDGFRa | Platelet derived growth factor receptor alpha; CD140a, | Biolegend | APA 5 | PE | 1:200 | 532nm (Green) | 575/26 | |
| N/A | ||||||||
| 562–588 nm |
*
Note: Dilutions shown should be used as a guideline for initial control experiments. The correct dilution needs to be determined for each antibody, fluorochrome, and the specific cytometer/sorter used for analysis, as laser power and the filters can influence the signal versus noise.