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(a) Immunofluorescence picture revealing the neuronal phenotype of PC12 cells after 5 days of NGF treatment. TH: tyrosine hydroxylase, as marker of dopamine; NFL: neurofilament-low-chain, as a marker of neuronal cytoskeleton. Nuclei were counterstained with DAPI. (b) Experimental set-up. CuE 10−10 M was administered 3 hours before MPP+ or DDC or bafilomycin (Baf) or rapamycin (Rap) treatment. CuE was administered once more 15 min after MPP+ or DDC or Baf or Rap.
