Table 1.
Fractional factorial design.
| Run order | Std order | PLGA (A) (mg) | PVA (B) (%w/v) | IEPR (C) | Speed (D) (rpm) | Time (E) (min) | Size (nm) | ZP (mV) | DE (%) |
|---|---|---|---|---|---|---|---|---|---|
| 1 | 7 | 50 | 2 | 6 | 7000 | 2 | 630.8 | −28.3 | 22.7 |
| 2 | 2 | 200 | 0.2 | 2 | 7000 | 2 | 357.3 | −24.1 | 19.64 |
| 3 | 6 | 200 | 0.2 | 6 | 7000 | 10 | 1058 | −29.8 | 13.58 |
| 4 | 3 | 50 | 2 | 2 | 7000 | 10 | 1014 | −23.7 | 15.90 |
| 5 | 4 | 200 | 2 | 2 | 10 000 | 2 | 744.7 | −27.7 | 7.28 |
| 6 | 5 | 50 | 0.2 | 6 | 10 000 | 2 | 423.8 | −27.1 | 7.65 |
| 7 | 1 | 50 | 0.2 | 2 | 10 000 | 10 | 329.9 | −22.2 | 4.44 |
| 8 | 8 | 200 | 2 | 6 | 10 000 | 10 | 387.1 | −38.2 | 10.09 |
|
| |||||||||
| One way ANOVA (P value) |
size | 0.87 | 0.51 | 0.95 | 0.17 | 0.49 | |||
| ZP | 0.75 | 0.55 | 0.67 | 0.06 | 0.34 | NA | |||
| DE | 0.80 | 0.51 | 0.004 | 0.60 | 0.74 | ||||
Zeta potential; DE: percentage drug encapsulation, IEPR: internal-external phase ratio.