Schematic of the An. gambiae 2LB-2LC sequence clusters and the co-orthologous regions of Ae. aegypti, and D. melanogaster. An. gambiae genes with light gray shading belong to sequence cluster 2LB and those with dark gray shading belong to sequence cluster 2LC (see text for details). Gene names are given in order along the chromosome, represented by horizontal black lines. For clarity, Ensembl gene names for Ae. aegyptii and An. gambiae are given without the full Ensembl prefixes AAEL and AGAP and leading zeroes. The positions of gene names above and below the line represent the gene orientation. Black lines connect putatively orthologous single-copy genes, whereas gray lines connect putatively co-orthologous groups. Genes with names in gray font do not code for cuticular proteins, whereas all other genes encode proteins of the RR-2 group.