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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Exp Eye Res. 2014 Nov 18;130:17–28. doi: 10.1016/j.exer.2014.11.010

Figure 1. Morphologies of rat ipRGCs.

Figure 1

A) Top row: Representative z-projected confocal images showing intracellular dye fills of the five ipRGC types. Arrows indicate axons. Bottom row: Rotated views of the regions outlined by rectangles in the above z-projections, showing the stratification patterns of distal dendrites. The ChAT bands (red) serve as markers for the S2 and S4 sublayers of the inner plexiform layer, which are in the OFF and ON sublaminas respectively. INL, inner nuclear layer; IPL, inner plexiform layer; GCL, ganglion cell layer. B,C) Plots of the total dendritic lengths, soma diameters, dendritic-field diameters and total branch points of all ipRGCs analyzed. The data points surrounded by squares correspond to the cells illustrated in panel A. D) Sholl analysis of dendritic branching patterns. Concentric circles were drawn at different distances from each cell’s soma center, and the number of dendrites crossing each circle was counted. The error bars represent S.E.M. N values: M1, 16; M2, 16; M3, 3; M4, 14; M5, 16.