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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Exp Eye Res. 2014 Nov 18;130:9–16. doi: 10.1016/j.exer.2014.11.009

Figure 2.

Figure 2

DEX treatment increases MYOC mRNA. RT-qPCR was performed on HTM27 cells treated with DEX or EtOH. Ct values were corrected for primer efficiency and compared to cells with no treatment as described by Pfaffl (Pfaffl, 2001). Data were then normalized to ITGB1 mRNA (β1 integrin housekeeping gene). (A) Levels of MYOC mRNA in the presence of DEX or EtOH for 1–6 days. (B) Levels of MYOC mRNA in HTM cells treated with DEX or EtOH for 6 days (Day 0) followed by 18 days with no DEX or EtOH. (C) Levels of MYOC mRNA in HTM cells treated with DEX or EtOH for 1 day only (Day 0) followed by 4 days with no DEX or EtOH. n = 3 biological replicates. DEX significantly different from EtOH *p<0.05, **p<0.04, ***p<0.02.