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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Exp Eye Res. 2014 Nov 18;130:9–16. doi: 10.1016/j.exer.2014.11.009

Figure 3.

Figure 3

CsA and INCA-6 inhibit the DEX-induced expression of MYOC mRNA. RT-qPCR was performed on 3 separate HTM cell lines (HTM17, HTM25 and HTM27). All cell strains had similar results so the data was averaged together. Individual cell strain data is shown in Table 1. Cells were pre-treated for 1 h with 1 or 10 μM CsA (A) or 40, 80 or 120 μM INCA-6 (B) prior to the addition of DEX or EtOH and incubated for 2 days. Fresh CsA, INCA-6, DEX and EtOH was added after 1 day. Ct values were corrected for primer efficiency and compared to cells with no treatment. Data were then normalized to ITGB1 mRNA (β1 integrin housekeeping gene). n = 4 biological replicates. DEX significantly different from DEX + 1 or 10 μM CsA (A) and from DEX + 40, 80 or 120 μM INCA-6 (B) *p<0.05.