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Ionomycin does not increase MYOC mRNA. RT-qPCR was performed on HTM27 cells treated with 1 or 5 μM ionomycin or DEX or EtOH for 2 days. Ct values were corrected for primer efficiency and compared to cells with no treatment as described by Pfaffl (Pfaffl, 2001). Data were then normalized to ITGB1 mRNA (β1 integrin housekeeping gene). n = 3 biological replicates.
