miRNA expression in HLHS patients. (A) Expression profile of miRNAs in non-failing HLHS pediatric patients by miRNA array. Green – down-regulated; Red – up-regulated. Only miRNAs with a qvalue of <0.025 are shown. miRNAs are presented by increasing qvalue. N=6 non-failing right ventricle (NF RV), 10 post-stage 1, 5 post-stage 3 HLHS patients. (B) Supervised hierarchical clustering of array samples based on Ward's method. (C) Relative expression of a subset of miRNAs in non-failing, post-stage 1 and post-stage 3 HLHS patients was confirmed by RT-PCR, as described in the Methods section. N=9 non-failing, 16 post-stage 1, 6 post-stage 3 HLHS patients. Statistically significant expression of miRNAs is shown in the Figure.