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. Author manuscript; available in PMC: 2016 Jan 31.
Published in final edited form as: Biochim Biophys Acta. 2014 Nov 1;1850(2):365–372. doi: 10.1016/j.bbagen.2014.09.029

FIGURE 2. Normalized pCa force relationship in skinned cardiac muscle fibers in the presence of PKA pseudo-phosphorylated cTnI.

FIGURE 2

The Ca2+ dependence of force development was measured in each preparation after cTnT displacement and binary complex reconstitution. In A) The pCa force relationship of fibers displaced with cTnT-WT and reconstituted with either cTnI-WT.cTnC (filled symbols) or PKA phosphorylation mimetic cTnI-SS/DD.cTnC complex (open symbols). Where in B) the skinned fibers were displaced with cTnT-ΔE96 and reconstituted with either cTnI-WT.cTnC (filled symbols) or the cTnI-SS/DD.cTnC (open symbols) complex. Data are expressed as mean ± S.E.