Table 1. Summary of pCa-force relationship curves before and after PKA incubation in fibers reconstituted with cardiac TnI.TnC complex at pH 7.0.

The pCa₅₀, nH and % Ca²⁺ unregulated force values are the average of many independent fiber experiments, and the errors are reported as S.E. values. The Ca²⁺ unregulated force was calculated using the following equation: $(F\text{pCa}8/F\text{pCa}4)\times 100$, where the FpCa8 and FpCa4 are the force at pCa 8.0 and pCa 4.0 solutions, respectively.

	Before PKA treatment		After PKA treatment
cTnT	pCa50	Hill coefficient, nH	pCa50	Hill coefficient, nH	ΔpCa50a	% Ca2+ Unregulated Force	N
cTnT-WT	5.73 ± 0.02	2.95 ± 0.08	5.47 ± 0.02b	3.73 ± 0.19b	−0.26	87.88 ± 4.95	6
cTnT–ΔE96	6.11 ± 0.02c	1.66 ± 0.05c	5.86 ± 0.03b,d	1.98 ± 0.17d	−0.25	96.19 ± 2.59	6

^aΔpCa50 = pCa₅₀ before PKA − pCa₅₀ after PKA

^bp<0.05 same cTnT before vs after PKA treatment

^cp<0.05: cTnT–ΔE96 vs cTnT-WT before PKA treatment

^dp<0.05: cTnT–ΔE96 vs cTnT-WT after PKA treatment