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. Author manuscript; available in PMC: 2016 Jan 1.
Published in final edited form as: Am J Transplant. 2014 Nov 17;15(1):64–75. doi: 10.1111/ajt.12999

Figure 2. H&E staining of native and decellularized kidneys.

Figure 2

Kidneys decellularized using Triton (C–D), Triton/SDS (E–F), or Trypsin-EGTA/Triton (G–H) were stained with H&E and compared to native kidneys (A–B). Decellularization with Triton resulted in retention of endothelial and smooth muscle cells in vessels and mesangial cells within the glomerulus. Triton/SDS and Trypsin-EGTA/Triton removed all cells as assessed by H&E. Representative images are shown at 10× (A, C, E, G) and 40× (B, D, F, H) magnification. Scale bars: 50 µm.