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. Author manuscript; available in PMC: 2015 Dec 22.
Published in final edited form as: Dev Cell. 2014 Dec 4;31(6):747–760. doi: 10.1016/j.devcel.2014.10.024

Figure 5. PLCγ is essential for PDGF chemotaxis and produces an asymmetric pattern of intracellular DAG.

Figure 5

A. Wind-rose plot of control cells migrating in a PDGF (n=67) gradient and loss of directional migration of PLCγ KD cells (n=70) in the same chamber.

B. Fibroblasts derived from PLCγ-null (PLCγ1-/-) (n=73) mice show a loss of chemotactic response to PDGF. Upon rescue of PLCγ expression, these fibroblasts regained their ability to chemotax (n=101).

C. Circular histogram of cells chemotaxing to PDGF gradients in a uniform concentration of Xestospongin-(c) (1 μM) (n=69).

D. Montage of an IA32 fibroblast transfected with GFP-tagged tandem C1 domain [(C1)2-GFP] chemotaxing to PDGF for more than five hours.

E. Enriched pixels in a chemotaxing cell are shown outlined in magenta. The intensity values of the segmented pixels are summed within angular bins at each time point to create a signaling “map” with the angle plotted on the horizontal axis and time on the vertical axis. Histogram showing the cumulative intracellular DAG distribution in chemotaxing cells expressing (C1)2-GFP (n=12 cells).

F. Enriched pixels in a randomly migrating cell are shown outlined in magenta. The intensity values of the segmented pixels are summed within angular bins at each time point to create a signaling “map” with the angle plotted on the horizontal axis and time on the vertical axis. Histogram showing the cumulative intracellular DAG distribution in randomly migrating cells expressing (C1)2-GFP (n=13 cells).