Figure 2.

Pharmacological profile of ML382 on MrgX1-expressing HEK293 cells. Dose response curve of ML382 in presence (red circles) and absence of BAM8-22 (black squares) determined by by Ca²⁺ imaging assay. EC₅₀ of ML382 in presence of submaximal amount of BAM8-22 (10 nM) is 190 nM. In the absence of BAM8-22, ML382 itself does not activate MrgX1. RFU: relative fluorescence units. The fitting was dose response fitting. The experiments were repeated three times. Error bars represent as Mean±SEM.^[11]