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. Author manuscript; available in PMC: 2015 Dec 24.
Published in final edited form as: J Renin Angiotensin Aldosterone Syst. 2014 Jun 24;16(4):1135–1148. doi: 10.1177/1470320313515039

Figure 8.

Figure 8

Influence of combined thimet oligopeptidase and neprilysin inhibition on Ang-(1–7) and Ang-(1–4) formation in isolated nuclei NRK-52E cells. Panel A: Metabolism of 125I-Ang I to Ang-(1–7) and Ang-(1–4) following 120 minute incubation with nuclei at 37°C. Panel B: Addition of thimet oligopeptidase inhibitor CPP (50 ZM) blocked the majority of Ang-(1–7) formation. Panel C: Addition of CPP and neprilysin inhibitor SCH39370 (SCH, 10 ZM). Panel D: Influence of CPP or CPP/SCH on the formation of Ang-(1–7) and Ang-(1–4) in nuclei at 120 minutes. All values represent mean ± SEM, n=3 from different cell passages; *P < 0.05 versus Control.