Skip to main content
. Author manuscript; available in PMC: 2015 Feb 5.
Published in final edited form as: Leukemia. 2014 Jun 25;29(2):474–482. doi: 10.1038/leu.2014.202

Fig. 3. Reduction of CSNK1α1 protein decrease MM cell survival and inhibit cell cycle progression.

Fig. 3

RPMI8226 cells were transduced with control vector or CSNK1α1 shRNAs as indicated. (A) CSNK1α1 was examined with immunoblotting of whole-cell lysates of Ctrl or shRNAs-transfected RPMI8226 cells. GAPDH served as a loading control. (B) Viability was assayed with MTT. Data represent triplicate cultures. (C) Cells were harvested at 72h post viral transduction and stained with PI. Cell cycle was analyzed with Flow Cytometry. (D) Annexin/PI staining and FACS analysis for apoptosis were performed on RPMI8226 cells transduced with control vector, sh3 or 5. Viable cells are represented in Q4 (lower left). Increasing percentages of apoptotic cells are represented in Q2 (upper right) and Q3 (Lower right).