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. Author manuscript; available in PMC: 2015 Feb 5.
Published in final edited form as: Leukemia. 2014 Jun 25;29(2):474–482. doi: 10.1038/leu.2014.202

Fig. 6. cMYC/KRAS12V cannot transform BaF3 cells independent of IL3 with low level of Csnk1α1.

Fig. 6

Fig. 6

(A) Schematic diagram of MSCV-based retroviral vectors: MK, MKCsnk1α1R1 and MKCsnk1α1R2. (B) Immunoblotting analysis shows expression of cMYC and KRAS12V in 293T cells transfected with the MK and MKCsnk1α1Rs vectors. GAPDH served as a loading control. (C) Immunoblotting shows Csnk1α1 expression in BaF3 cells transfected with MK and MKCsnk1α1Rs vectors. GAPDH served as a loading control. (D) Photographs of BaF3 cells transduced with MK, MKCsnk1α1R1, or MKCsnk1α1R2 vectors. MKCsnk1α1R1 transfection cannot drive BaF3 cell growth independent of IL3 in vitro (middle).