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. 2014 Dec 1;6(11):931–942. doi: 10.18632/aging.100702

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Copyright: © 2014 Bernal et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited

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(A and C) Immunoblots, using the indicated antibody, in tissue lysate from Nfkb1^+/+ mouse brain (A) and liver (C) at 1 and 18 months of age. n=3 animals per group. (B and D) EMSA in tissue lysate from Nfkb1^+/+ mouse brain (B) and liver (D) at 1 and 18 months of age. Supershift (SS) was performed with the indicated antibodies. n=3 animals per group. N-non-specific and S-specific cold competitors verify the NF-(B bands. NS-non-specific bands. (E) Immunoblot using the indicated antibodies in primary Nfkb1^+/+ MEFs at passage 2, 4, and 6. n=3 each lane represents a separate MEF isolate.