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α-synuclein did not accumulate in ATP13A2 deficient SH-SY5Y cells. (A-C) Westernblot analysis of α-synuclein. Human α-synuclein was overexpressed both in the WT and ATP13A2 -/- SH-SY5Y cells. On day 3 after infection, the levels of α-synuclein was measured in triton X-100 soluble (B) and insoluble fraction (C). ATP13A2 depletion in SH-SY5Y cell did not change the level of α-synuclein. n=3.
